IN VITRO EVALUATION OF ANTI-BIOFILM POTENTIAL OF ALUM AND ITS BHASMA (SPHATIKA BHASMA)

Microbial biofilms may form on wide variety of surfaces and are problematic in medical, industrial environmental settings. Natural antibiofilm agents promising candidates for treatment biofilm-associated infections. One such natural products used therapeutic purposes is Alum (Sphatika). Besides, Ayurvedic Bhasmas various ailments India. In the present vitro study, anti-Biofilm potential as well its Bhasma- Sphatika Bhasma was evaluated against total 8 bacteria, viz., 4 gram negative positive using crystal violet staining method. Antibiofilm activity noted order P. aeruginosa > S. lutea E. coli K. pneumoniae typhi MRSA B. subtilis faecalis, whereas, effect found to be faecalis. general, exhibited better Anti-Biofilm than Alum. Furthermore, four different combinations prepared from (AL), (SB) Ciprofloxacin (CP)- standard antibiotic were their combinatorial bacterial biofilms. combination (AL + SB) displayed highest Anti-biofilm all effects could attributed Flavonoid content. Moreover, best our knowledge, this first study revealing anti-biofilm Bhasma.